Identification of 14-3-3zeta by chemical affinity with salicylanilide inhibitors of interleukin-12p40 production.
Bioconjug Chem
; 19(9): 1775-84, 2008 Sep.
Article
em En
| MEDLINE
| ID: mdl-18763820
ABSTRACT
Salicylanilides were found as selective inhibitors of interleukin-12p40 production in stimulated dendritic cells. The conversion of one of these bioactive salicylanilides into a comparably bioactive, chemically labeled derivative was achieved using a facile and systematic functional group derivatization strategy. This resulted in a tool reagent that was then employed in an affinity chromatography approach that resulted in the identification of the protein 14-3-3zeta as having selective affinity for the chromatography matrix that was derivatized with a salicylanilide that inhibited IL-12p40 production.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salicilanilidas
/
Marcadores de Afinidade
/
Proteínas 14-3-3
/
Subunidade p40 da Interleucina-12
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article