Phenotypic analysis for a cell line of canine epidermal keratinocytes.
J Vet Med Sci
; 70(8): 853-5, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18772565
ABSTRACT
Epidermal keratinocytes have the potential to produce inflammatory mediators that are considered to play an important role in skin diseases such as atopic dermatitis (AD). Thus, cell lines of canine epidermal keratinocytes are useful for studying the biological reactivity of keratinocytes in vitro. However, there has been no report on properly analyzing the phenotype of canine keratinocyte cell lines. In this work, we performed phenotypic analysis of CPEK, which was derived from the epidermis of an adult dog in order to examine the phenotypic similarity with epidermal keratinocytes. The present findings indicated that CPEK cells expressed markers for epidermal keratinocytes including cytokeratin 14, alpha6 integrin and PCNA. Our findings demonstrated that CPEK could be a useful cell line for investigating the central role of epidermal keratinocytes in the pathogenesis of AD in vitro.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
/
Dermatite Atópica
/
Doenças do Cão
/
Cães
/
Epiderme
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article