Glucose homeostasis across human airway epithelial cell monolayers: role of diffusion, transport and metabolism.
Pflugers Arch
; 457(5): 1061-70, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-18781323
ABSTRACT
Glucose in airway surface liquid (ASL) is maintained at low concentrations compared to blood glucose. Using radiolabelled [(3)H]-D -glucose and [(14)C]-L -glucose, detection of D - and L -glucose by high-performance liquid chromatography and metabolites by nuclear magnetic resonance, we found that glucose applied to the basolateral side of H441 human airway epithelial cell monolayers at a physiological concentration (5 mM) crossed to the apical side by paracellular diffusion. Transepithelial resistance of the monolayer was inversely correlated with paracellular diffusion. Appearance of glucose in the apical compartment was reduced by uptake of glucose into the cell by basolateral and apical phloretin-sensitive GLUT transporters. Glucose taken up into the cell was metabolised to lactate which was then released, at least in part, across the apical membrane. We suggest that glucose transport through GLUT transporters and its subsequent metabolism in lung epithelial cells help to maintain low glucose concentrations in human ASL which is important for protecting the lung against infection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Epiteliais
/
Glucose
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article