Study of the identification of enterotoxigenic Escherichia coli by LT-DNA gene hybridization.
Chin Med J (Engl)
; 104(1): 14-7, 1991 Jan.
Article
em En
| MEDLINE
| ID: mdl-1879189
ABSTRACT
Reference strains of enterotoxigenic Escherichia coli (ETEC), non-ETEC, and other enteropathogenic bacteria were used to prove the reliability of LT-DNA gene hybridization. In the test, LT-DNA gene hybridization was compared with plate immunohemolytic test (PIHT) in identifying LT-ETEC. The results obtained by both methods showed no significant differences. 791 strains of E. coli isolated from 1,875 children with acute diarrhea in Taiyuan Children's Hospital were examined for LT-ETEC by LT-DNA gene hybridization and PIHT. 289 strains examined by LT-DNA gene hybridization were LT positive, while 205 strains examined by PIHT were LT positive. Three different assays were done colony hybridization, PIHT and fecal direct blot hybridization on each of 74 fecal specimens from children with acute diarrhea. It was found that identification of LT-ETEC using fecal direct blot hybridization is a simple, sensitive and more practical method.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
DNA Bacteriano
/
Proteínas de Escherichia coli
/
Diarreia
/
Enterotoxinas
/
Escherichia coli
Tipo de estudo:
Diagnostic_studies
Limite:
Child, preschool
/
Humans
/
Infant
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article