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Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.
Olmedo-Verd, Elvira; Valladares, Ana; Flores, Enrique; Herrero, Antonia; Muro-Pastor, Alicia M.
Afiliação
  • Olmedo-Verd E; Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas, Universidad deSevilla, E-41092 Seville, Spain.
J Bacteriol ; 190(22): 7584-90, 2008 Nov.
Article em En | MEDLINE | ID: mdl-18805988
Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N(2) in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene during heterocyst differentiation was studied in vivo by using an ntcA-gfp translational fusion and primer extension analysis. Mutation of neither of the two identified NtcA-binding sites eliminated localized expression of ntcA in proheterocysts, but mutation of both sites led to very low, nonlocalized expression.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Regiões Promotoras Genéticas Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Regiões Promotoras Genéticas Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2008 Tipo de documento: Article