[Effect of rosiglitazone on tumor necrosis factor-alpha-induced nuclear factor-kappaB and coupling factor 6 expressions in human umbilical vein endothelial cells].
Nan Fang Yi Ke Da Xue Xue Bao
; 28(9): 1642-5, 2008 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-18819888
ABSTRACT
OBJECTIVE:
To investigate the effect of rosiglitazone on the expression of nuclear factor-kappaB (NF-kappaB) and coupling factor 6 (CF6) induced by tumor necrosis factor-alpha (TNF-alpha) in cultured human umbilical vein endothelial cells (HUVEC).METHODS:
Cultured HUVEC of passage 3-5 were stimulated with TNF-alpha and then cultured in the presence of rosiglitazone. The expression of CF6 and NF-kappaB subunit p65 were evaluated by immunocytochemistical method.RESULTS:
Pretreatment of HUVECs with rosiglitazone inhibited TNF-alpha-induced expression of CF6 in a dose-dependent manner. The activation of CF6 stimulated by TNF-alpha was suppressed by ROS in a dose-dependent manner.CONCLUSION:
TNF-alpha-induced enhancement of the gene expression and release of CF6 is mediated by activation of NF-kappaB signaling pathway. ROS can inhibit the activation of IKK, block NF-kappaB signaling pathway and inhibit the expression of CF6, which may be the mechanism underlying the action of TZDs on hypertension.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores Acopladores da Fosforilação Oxidativa
/
NF-kappa B
/
Fator de Necrose Tumoral alfa
/
ATPases Mitocondriais Próton-Translocadoras
/
Células Endoteliais
/
Tiazolidinedionas
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article