[Effect of cryopreservation of bone marrow stromal cells on periodontal tissue regeneration].

OBJECTIVE: To investigate the influence of cryopreservation on bone marrow stromal cells' (BMSC) capability of enhancing periodontal regeneration. METHODS: Twenty-six artificial periodontal defects were established in 5 Beagle dogs and divided into 3 groups at random. Only collagen membrane, the complex of cryopreserved and un-cryopreserved BMSC and collagen scaffold were transplanted in the blank control group, cryopreserved and un-cryopreserved groups respectively. The periodontal regeneration was observed 8 weeks after transplantation. RESULTS: The percentage of periodontal regeneration in cryopreserved and un-cryopreserved groups was significantly greater than that of the blank control group (P < 0.05), but no statistical difference was found between cryopreserved and un-cryopreserved groups. CONCLUSIONS: Cryopreservation had no significant negative effects on BMSC capability of enhancing periodontal regeneration.