Technical considerations in using DNA microarrays to define regulons.
Methods
; 47(1): 63-72, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-18955146
ABSTRACT
Transcription is the major regulatory target of gene expression in bacteria, and is controlled by many regulatory proteins and RNAs. Microarrays are a powerful tool to study the regulation of transcription on a genomic scale. Here we describe the use of transcription profiling and ChIP-chip to study transcriptional regulation in bacteria. Transcription profiling determines the outcome of regulatory events whereas ChIP-chip identifies the protein-DNA interactions that determine these events. Together they can provide detailed information on transcriptional regulatory systems.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Regulação Bacteriana da Expressão Gênica
/
Regulon
/
Análise de Sequência com Séries de Oligonucleotídeos
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article