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Determination of vitamin D(3) hydroxymetabolites in plasma at the sub-part per trillion levels using on-line cleanup/preconcentration and HPLC-fluorimetric post-column derivatisation.
Ortiz Boyer, F; Fernández Romero, J M; Luque de Castro, M D; Quesada, J M.
Afiliação
  • Ortiz Boyer F; Analytical Chemistry Division, Faculty of Sciences, University of Córdoba, Córdoba E-4004, Spain.
Talanta ; 50(1): 57-66, 1999 Aug 23.
Article em En | MEDLINE | ID: mdl-18967694
ABSTRACT
A new method for the determination of the hydroxymetabolites of vitamin D(3) (24,25-(OH)(2)-D(3), 1,25-(OH)(2)-D(3) and 25-OH-D(3)) in plasma is reported. The method is based on the integration of three subsystems continuous cleanup/preconcentration, HPL separation and post-column fluorimetric derivatisation. The derivatising subsystem is based on the dehydration reaction undergone by the secosteriod molecules in a strong-acid medium. The calibration graphs were run between 0.1 pg ml(-1) and 100 ng ml(-1) for each analyte with excellent regression coefficients (>/=0.9933) in all cases. The precision at two concentration levels was established with acceptable RSDs (%) in all instances (values between 2.1 and 5.2%). The method was also checked by applying it to human plasma samples spiked with the target analytes and the recoveries ranged between 86 and 106%.
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Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 1999 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 1999 Tipo de documento: Article