Cdc42, Par6, and aPKC regulate Arp2/3-mediated endocytosis to control local adherens junction stability.
Curr Biol
; 18(21): 1631-8, 2008 Nov 11.
Article
em En
| MEDLINE
| ID: mdl-18976918
ABSTRACT
BACKGROUND:
By acting as a dynamic link between adjacent cells in a monolayer, adherens junctions (AJs) maintain the integrity of epithelial tissues while allowing for neighbor exchange. Although it is not currently understood how this combination of AJ stability and plasticity is achieved, junctionally associated actin filaments are likely to play a role, because actin-based structures have been implicated in AJ organization and in the regulation of junctional turnover.RESULTS:
Here, through exploring the role of actin cytoskeletal regulators in the developing Drosophila notum, we have identified a critical role for Cdc42-aPKC-Par6 in the maintenance of AJ organization. In this system, the loss or inhibition of Cdc42-aPKC-Par6 leads to junctional discontinuities, the formation of ectopic junctional structures, and defects in apical actin cytoskeletal organization. Affected cells also undergo progressive apical constriction and, frequently, delamination. Surprisingly, this Cdc42-aPKC-Par6-dependent regulation of junctional stability was found to be independent of several well-known targets of Cdc42-aPKC-Par6 Baz, Lgl, Rac, and SCAR. However, similar AJ defects are observed in wasp, arp2/3, and dynamin mutant cells, suggesting a requirement for actin-mediated endocytosis in the maintenance of junctional stability downstream of Cdc42. This was confirmed in endocytosis assays, which revealed a requirement for Cdc42, Arp2/3, and Dynamin for normal rates of E-cadherin internalization.CONCLUSIONS:
By focusing on the molecular mechanisms required to maintain an epithelium, this analysis reveals a novel role for the epithelial polarity machinery, Cdc42-Par6-aPKC, in local AJ remodeling through the control of Arp2/3-dependent endocytosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína Quinase C
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Caderinas
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Proteína cdc42 de Ligação ao GTP
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Junções Aderentes
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Proteínas de Drosophila
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Drosophila
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Endocitose
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article