The effect of volume of chorionic villi on long-term cell culture.

ABSTRACT

OBJECTIVE: It was the aim of our study to investigate the association between culture time and weight of villi obtained by transabdominal chorionic villus sampling (CVS). METHODS: We analyzed 1,442 villus samples. RESULTS: The gestational age at sampling ranged from 10 to 14 weeks. The weight of villi in these samples ranged from 1 to 80 mg (median 10 mg, interquatile range 7-12 mg). The culture time ranged from 5 to 24 days. Culture time was significantly and inversely correlated with the weight of villi obtained (r = -0.258, p < 0.01). Time was significantly longer when 5 mg or less of villi was obtained but the difference was only up to 2 days. CONCLUSIONS: Because the difference in culture time for different groups of villus samples was within 1-2 days, this relationship is statistically significant but has no clinical significance.