Purification and characterization of recombinant lipid storage protein-2 from Drosophila melanogaster.
Protein Pept Lett
; 15(9): 1027-32, 2008.
Article
em En
| MEDLINE
| ID: mdl-18991782
ABSTRACT
Lipid storage protein 2 (Lsd 2) is a conserved insect protein that belongs to the small PAT family of proteins. PAT proteins are found associated to the lipid droplets of adipocytes and play significant roles in the regulation of triacylglycerides metabolism. Here we describe the expression and purification of Lsd2, its reconstitution in lipoprotein particles, the location of putative lipid binding sites and its secondary structure. This study provides the starting point for future studies on the mechanism of function of Lsd2. The similarities and differences between Lsd1 and Lsd2, the only PAT proteins found in insects, are discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Drosophila
/
Drosophila melanogaster
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article