Methods for the analysis of HIV-1 nucleocapsid protein interactions with oligonucleotides.
Methods Mol Biol
; 485: 209-21, 2009.
Article
em En
| MEDLINE
| ID: mdl-19020828
ABSTRACT
HIV-1 Nucleocapsid protein (NC) is a small basic protein that contains two retroviral zinc fingers. It is a highly effective nucleic acid chaperone that plays a critical role in viral replication acting as a cofactor in reverse transcription as well as other aspects of the viral lifecycle. We have used a variety of biophysical techniques to characterize the high affinity binding of NC to a short deoxyoligonucleotide (d(TG)(4)). Here we outline in detail the use of fluorescence anisotropy and surface plasmon resonance spectroscopy to study the binding of NC to d(TG)(4).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
HIV-1
/
Produtos do Gene gag do Vírus da Imunodeficiência Humana
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article