Different epithelial cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis.
J Cataract Refract Surg
; 34(12): 2079-84, 2008 Dec.
Article
em En
| MEDLINE
| ID: mdl-19027563
ABSTRACT
PURPOSE:
To determine the anatomic cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis (epi-LASIK).SETTING:
Department of Ophthalmology, IIsan Paik Hospital, Goyang, Korea.METHODS:
Sixteen eyes (8 patients) were included in this study. Three epikeratomes, the Moria Epi-K, Centurion SES, and Amadeus II, were used to collect 4 epithelial flaps from 2 patients in the epi-LASIK procedure. Four epithelial flaps from 2 patients were also obtained by laser-assisted subepithelial keratectomy (LASEK). Immunohistochemical staining using monoclonal antibodies against integrin beta1, integrin beta4, laminin 5, and collagen type VII was performed.RESULTS:
Immunohistochemical staining showed expression of integrin beta1 and integrin beta4 in all epithelial flaps. In epi-LASIK, the expression of laminin 5 and collagen type VII had a linear or dotted pattern that differed based on the epikeratome used. In the epithelial flaps obtained using LASEK, the expression of laminin 5 and collagen type VII had a dotted pattern.CONCLUSIONS:
Each epikeratome yielded reproducible but different cleavage planes of corneal epithelium. The results suggest that further study is needed to elucidate the wound-healing process after epi-LASIK because different cleavage planes produced by different epikeratomes may influence the process.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retalhos Cirúrgicos
/
Epitélio Corneano
/
Ceratomileuse Assistida por Excimer Laser In Situ
/
Ceratectomia Subepitelial Assistida por Laser
/
Lasers de Excimer
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article