Combining subproteome enrichment and Rubisco depletion enables identification of low abundance proteins differentially regulated during plant defense.
Proteomics
; 9(1): 138-47, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-19053141
ABSTRACT
Transgenic Arabidopsis conditionally expressing the bacterial avrRpm1 type III effector under the control of a dexamethasone-responsive promoter were used for proteomics studies. This model system permits study of an individual effector without interference from additional bacterial components. Coupling of different prefractionation approaches to high resolution 2-DE facilitated the discovery of low abundance proteins - enabling the identification of proteins that have escaped detection in similar experiments. A total of 34 differentially regulated protein spots were identified. Four of these (a remorin, a protein phosphatase 2C (PP2C), an RNA-binding protein, and a C2-domain-containing protein) are potentially early signaling components in the interaction between AvrRpm1 and the cognate disease resistance gene product, resistance to Pseudomonas syringae pv. maculicola 1 (RPM1). For the remorin and RNA-binding protein, involvement of PTM and post-transcriptional regulation are implicated, respectively.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Arabidopsis
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Regulação da Expressão Gênica de Plantas
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Proteínas de Arabidopsis
/
Proteômica
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article