Detection of human spermatid-specific transcripts in peripheral blood lymphocytes of males and females.
Hum Genet
; 87(3): 307-10, 1991 Jul.
Article
em En
| MEDLINE
| ID: mdl-1907596
ABSTRACT
We describe the detection of ectopic ("illegitimate") transcripts of the proacrosin and protamine 2 genes, which are specific for human spermatogenesis, in non-cultured peripheral blood lymphocytes. After specifically-primed reverse transcription of total lymphocyte RNA, these rare transcripts can be directly visualised after two rounds of polymerase chain reaction with nested primers. Sequence and restriction analyses of the corresponding fragments have confirmed that transcripts of proacrosin and protamine 2 are present in the lymphocytes not only of males, but also of adult females.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermátides
/
RNA Mensageiro
/
Acrosina
/
Linfócitos
/
Protaminas
/
Precursores Enzimáticos
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article