MCF-7 breast carcinoma cells express ryanodine receptor type 1: functional characterization and subcellular localization.
Mol Cell Biochem
; 323(1-2): 39-47, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-19082546
ABSTRACT
Breast carcinoma-derived MCF-7 cells are frequently used in biomedical research. However, few reports exist regarding the characterization of signaling mechanisms in these cancerous cells involved in intracellular Ca(2+) dynamics. Consequently, the aim of these experiments was to characterize the ryanodine receptor/Ca(2+) release channel (RyR) present in MCF-7 cells. Ryanodine (100 nM), cADPR (5 microM), and caffeine (10 mM) promoted cytoplasmic Ca(2+) mobilization; in contrast, ryanodine at inhibitory concentration (100 microM) decreased the basal Ca(2+) level. Fluorescent probes demonstrated that RyR is located mainly in endomembranes. Some degree of co-localization with inositol trisphosphate receptor (IP(3)R) was observed, whereas coincidence with thapsigargin-sensitive Ca(2+)-ATPase (SERCA) was more limited. Molecular cloning resulted in the detection exclusively of RyR isoform 1. For the first time, it is shown that MCF-7 cells express functional RyR.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Canal de Liberação de Cálcio do Receptor de Rianodina
/
Isoformas de Proteínas
Limite:
Aged
/
Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article