Friend murine leukemia virus A8 regulates Env protein expression through an intron sequence.
Virology
; 385(1): 115-25, 2009 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-19108860
ABSTRACT
Friend murine leukemia virus (Fr-MLV) produces unspliced mRNAs, as well as singly-spliced mRNAs by which the Env protein is generated. We investigated the role of the intron within the Fr-MLV gene in Env expression using vectors with serially truncated introns. The up-regulatory regions, the 361-878 and the 5135-5399 fragments, and the down-regulatory regions, the 1904-2849 and the 3995-4287 fragments, were found to influence the splicing efficiency. The Env protein expression level was proportional to the amount of env-mRNA. Furthermore, we found that the splicing process is important to acquire env-mRNA stability, and it also promotes translation of the Env protein. These splicing-dependent phenomena were not observed when luciferase expression vectors were analyzed. These findings indicated that the Env protein expression level in MLV is regulated at multiple steps:
the env-mRNA expression level is determined by the splicing efficiency, and the stability of env-mRNA is acquired through the splicing process.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação Viral da Expressão Gênica
/
Produtos do Gene env
/
Vírus da Leucemia Murina de Friend
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article