AtAzg1 and AtAzg2 comprise a novel family of purine transporters in Arabidopsis.
FEBS Lett
; 583(2): 481-6, 2009 Jan 22.
Article
em En
| MEDLINE
| ID: mdl-19121308
ABSTRACT
In plants, nucleobase biochemistry is highly compartmented relying upon a well-regulated and selective membrane transport system. In Arabidopsis two proteins, AtAzg1 and AtAzg2, show substantial amino acid sequence similarity to the adenine-guanine-hypoxanthine transporter AzgA of Aspergillus nidulans. Analysis of single and double mutant lines harboring T-DNA insertion alleles AtAzg1-1 and AtAzg2-1 reveal a marked resistance to growth in the presence of 8-azaadenine and 8-azaguanine but not to other toxic nucleobase analogues. Conversely, yeast strains expressing AtAzg1 and AtAzg2 gain heightened sensitivity to growth on 8-azaadenine and 8-azaguanine. Radio-labeled purine uptake experiments in yeast and in planta confirm the function of AtAzg1 and AtAzg2 as plant adenine-guanine transporters.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adenina
/
Arabidopsis
/
Proteínas de Transporte de Nucleobases
/
Guanina
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article