[Molecular epidemiological study on norovirus among children with acute diarrhea in Guangzhou].
Zhonghua Er Ke Za Zhi
; 46(12): 899-904, 2008 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-19134251
ABSTRACT
OBJECTIVE:
To study molecular epidemiology of norovirus (NV) infections, stool specimens collected from children with acute diarrhea were tested by TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR) for the viral specific nucleic acid segments.METHODS:
Fecal samples from a total of 1260 children who had watery diarrhea seen from December 2006 to December 2007 in Guangzhou were analyzed by real-time RT-PCR. The primers and probes used for rapid detection and typing of NV strain target NV sequences were at the ORF1-ORF2 junction, a highly conserved region of the NoV genome. The positive specimens were determined by nested PCR and sequenced.RESULTS:
Totally 257 specimens were positive for NV with a positive rate of 20.40%. Shedding of NV type GI was detected in 6.90%, type GII in 16.98% respectively, while the positive number of mixed infection with GI and GII was 44. Of the NV strains that were cloned and sequenced, GI was GI-3, GI-2 and GI-4 detected in positive specimens respectively; meanwhile, GII-4 was most commonly seen in genome II, followed by GII-3 and GII-7. In addition, the average age of children infected with NV was less than 2 years. An epidemic occurred during the winter and early spring (December through the next March).CONCLUSION:
NV was one of the important pathogens for acute diarrhea among children in Guangzhou, which suggested GII-4 was the prevalent strain.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Caliciviridae
/
Norovirus
/
Diarreia
/
Fezes
Tipo de estudo:
Etiology_studies
Limite:
Child, preschool
/
Humans
/
Infant
País/Região como assunto:
Asia
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article