Identification of Staphylococcus species directly from positive blood culture broth by use of molecular and conventional methods.
J Clin Microbiol
; 47(4): 1082-6, 2009 Apr.
Article
em En
| MEDLINE
| ID: mdl-19213701
ABSTRACT
We compared two real-time PCR assays (both by the use of melting curve analysis) for their ability to identify Staphylococcus species directly from 200 positive blood culture bottles. The PCR assays correctly identified 83% to 94% of the Staphylococcus isolates to species clusters. Molecular testing significantly outperformed commercially available latex tests (sensitivity for both latex tests, <15%) when it was used directly with broth from signal-positive blood cultures.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Estafilocócicas
/
Staphylococcus
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Sangue
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Reação em Cadeia da Polimerase
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Técnicas Bacteriológicas
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article