Molecular evidence for tick-borne encephalitis virus in ticks in South Korea.
Med Vet Entomol
; 23(1): 15-20, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-19239610
ABSTRACT
To investigate whether tick-borne encephalitis viruses (TBEVs) are present in South Korea, Korean ixodid ticks were tested for TBEV RNA. Ticks (n = 2460) were collected from wild and domestic animals or by flagging at forest and grassland in 12 regions of five provinces in 2005-06. Four species in two genera were identified, yielding 197 sample pools (1-20 ticks per pool); from these, 12 envelope protein gene fragments of TBEV were amplified by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR). Ten of the 2104 adult ticks (0.4%) and two of the 356 nymph ticks (0.2%) were positive for the envelope (E) gene of TBEV. Twelve TBEV RNA-positive samples were detected in Gyeonggi and Gangwon provinces. Phylogenetic analysis showed that the E genes of the TBEV isolates were clustered with the Western European subtype (98% identity). This study suggests that TBEVs may exist in Korea.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ixodidae
/
Vírus da Encefalite Transmitidos por Carrapatos
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article