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Reappraisal of HLA antibody analysis and crossmatching in kidney transplantation.
Lee, P-C; Ozawa, M; Hung, C-J; Lin, Y-J; Chang, S-S; Chou, T-C.
Afiliação
  • Lee PC; Medical College, National Cheng Kung University, Tainan, Taiwan. pochang@mail.ncku.edu.tw
Transplant Proc ; 41(1): 95-8, 2009.
Article em En | MEDLINE | ID: mdl-19249487
Enzyme-linked immunosorbent assay (ELISA) and flow cytometric techniques have been introduced to overcome the limited sensitivity and specificity of the CDC assay. This retrospective study used lambda antigen tray-mixed screening and Luminex HLA class I and II specificity assays to re-examine: (1) the accuracy with which detection of HLA antibody and specificity by ELISA predicts pretransplantation National Institutes of Health (NIH)/Centers for Disease Control and Prevention (CDC) crossmatch; and (2) a comparison of Luminex and ELISA methods to detect HLA antibodies. Sera from 481 patients awaiting kidney transplantation were tested using the ELISA method lambda antigen tray-mixed and using NIH-CDC to determine how well HLA antibodies detected using ELISA predicted crossmatches using CDC. Pretransplantation sera from 48 patients with follow-up data were retested using both ELISA lambda antigen tray-mixed and Luminex to compare the efficacy of the 2 methods.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Teste de Histocompatibilidade / Transplante de Rim / Antígenos HLA / Isoanticorpos Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Teste de Histocompatibilidade / Transplante de Rim / Antígenos HLA / Isoanticorpos Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Ano de publicação: 2009 Tipo de documento: Article