[In vivo effect of recombined IL-15/Fc fusion protein on EAU].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 39(6): 944-9, 2008 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-19253832
ABSTRACT
OBJECTIVE:
To test the effect of recombined IL-15/Fc on experimental autoimmune uveitis (EAU) in mice.METHODS:
EAU were induced in C57 mice by transferring activated T cells specific to the interphotoreceptor-binding protein (IRBP) 1-20 peptide. The mice were then treated with recombine IL-15/Fc fusion protein or IgG as controls. The severity of EAU were graded on a scale of 0 to 4 with half-point increment based on the type, number, and size of the lesions detected by funduscopic and HE staining. The IRBP1-20 sensitive CD8+T cells were isolated from the IRBP1-20 immune mice with auto-MACS. The in vitro effect of IL-15/Fc fusion protein on the proliferation, differentiation, expansion and production of inflammatory cytokines of the purified IRBP1-20 sensitive CD8+T cells were analyzed with 3HTdR, FACS and ELISA.RESULTS:
IL-15/Fc fusion protein inhibited the activation, proliferation, expansion and production of inflammatory cytokines of the IRBP1-20 specific CD8+T cells, down regulated CD44(high)CD62L(low) effect and CD8+ CD62L(low) activated T cell subsets, and consequently decreased the severity of EAU.CONCLUSION:
IL-15/Fc fusion proteins decrease the severity of EAU through inhibiting the proliferation, expansion, differentiation and production of inflammatory cytokines of CD8+ T cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças Autoimunes
/
Uveíte
/
Proteínas Recombinantes de Fusão
/
Linfócitos T CD8-Positivos
/
Interleucina-15
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article