Mitochondrial genome DNA analysis of the domestic dog: identifying informative SNPs outside of the control region.
J Forensic Sci
; 54(2): 275-88, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-19261050
ABSTRACT
While the mitochondrial control region has proven successful for human forensic evaluations by indicating ethnic origin, domestic dogs (Canis lupus familiaris) of seemingly unrelated breeds often form large groups based on identical control region sequences. In an attempt to break up these large haplotype groups, we have analyzed the remaining c. 15,484 base pairs of the canine mitochondrial genome for 79 dogs and used phylogenetic and population genetic methods to search for additional variability in the form of single nucleotide polymorphisms (SNPs). We have identified 356 SNPs and 65 haplotypes in the remainder of the mitochondrial genome excluding the control region. The exclusion capacity was found to be 0.018. The mitochondrial control region was also evaluated for the same 79 dogs. The signals from the different fragments do not conflict, but instead support one another and provide a larger fragment of DNA that can be analyzed as forensic evidence.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
/
Polimorfismo de Nucleotídeo Único
/
Cães
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article