An electrochemical biosensor array for rapid detection of alanine aminotransferase and aspartate aminotransferase.
Biosci Biotechnol Biochem
; 73(3): 474-8, 2009 Mar 23.
Article
em En
| MEDLINE
| ID: mdl-19270406
An increment of alanine aminotransferase (ALT) or aspartate aminotransferase (AST) in human serum indicates an abnormal symptom of the liver. Hence, an electrochemical biosensor array that uses micro electro mechanical systems technology is required for rapid and integrated measurement of ALT/AST. Here we describe a biosensor array consisting of two glutamate sensors. It turned out that porous silicon layers formed on each working electrode were useful to increase the effective surface area. This biosensor array was constructed with platinum electrodes and a polydimethylsiloxane (PDMS) microchannel. Electrodes in sampling wells minimized a cross-interference effect and permitted multiple sampling by immobilization with glutamate oxidase using a silanization technique. The device sensitivities derived from semi-logarithmic plots were 0.145 microA/(U/l) for ALT and 0.463 microA/(U/l) for AST over a range of 1.3 U/l to 250 U/l. Hene, this ALT/AST biosensor array can be applied in diagnostic and home use.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aspartato Aminotransferases
/
Técnicas Biossensoriais
/
Alanina Transaminase
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article