Potentiation of angiogenesis and regeneration by G-CSF after sciatic nerve crush injury.
Biochem Biophys Res Commun
; 382(1): 177-82, 2009 Apr 24.
Article
em En
| MEDLINE
| ID: mdl-19275877
ABSTRACT
Granulocyte colony-stimulating factor (G-CSF) demonstrates neuroprotective effects through different mechanisms, including mobilization of bone marrow cells. However, the influence of G-CSF-mediated mobilization of bone marrow-derived cells on injured sciatic nerves remains to be elucidated. The administration of G-CSF promoted a short-term functional recovery 7 days after crush injury in sciatic nerves. A double-immunofluorescence study using green fluorescent protein-chimeric mice revealed that bone marrow-derived CD34+ cells were predominantly mobilized and migrated into injured nerves after G-CSF treatment. G-CSF-mediated beneficial effects against sciatic nerve injury were associated with increased CD34+ cell deposition, vascular endothelial growth factor (VEGF) expression, and vascularization/angiogenesis as well as decreased CD68+ cell accumulation. However, cell differentiation and VEGF expression were not demonstrated in deposited cells. The results suggest that the promotion of short-term functional recovery in sciatic nerve crush injury by G-CSF involves a paracrine modulatory effect and a bone marrow-derived CD34+ cell mobilizing effect.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nervo Isquiático
/
Fator Estimulador de Colônias de Granulócitos
/
Fármacos Neuroprotetores
/
Neovascularização Fisiológica
/
Regeneração Nervosa
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article