The SUMO-E3 ligase PIAS3 targets pyruvate kinase M2.
J Cell Biochem
; 107(2): 293-302, 2009 May 15.
Article
em En
| MEDLINE
| ID: mdl-19308990
ABSTRACT
Pyruvate kinase M2 (M2-PK) controls the rate-limiting step at the end of the glycolytic pathway in normal proliferating and tumor cells. Other functions of M2-PK in addition to its role in glycolysis are little understood. The aim of this study was to identify new cellular interaction partners of M2-PK in order to discover novel links between M2-PK and cellular functions. Here we show that the SUMO-E3 ligase protein PIAS3 (inhibitor of activated STAT3) physically interacts with M2-PK and its isoenzyme M1-PK. Moreover, we demonstrate that endogenous SUMO-1-M2-PK conjugates exist in mammalian cells. Furthermore, we show that transient expression of PIAS3 but not the RING domain mutant PIAS3 (C299S, H301A) is consistent with nuclear localization of M2-PK and PIAS3 and M2-PK partially co-localize in the nucleus of these cells. This study suggests a link between PIAS3 and nuclear pyruvate kinase.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piruvato Quinase
/
Transdução de Sinais
/
Chaperonas Moleculares
/
Proteínas Inibidoras de STAT Ativados
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article