Digital ion trap mass spectrometer for probing the structure of biological macromolecules by gas phase X-ray scattering.
Anal Chem
; 81(9): 3392-7, 2009 May 01.
Article
em En
| MEDLINE
| ID: mdl-19354287
ABSTRACT
Small-angle X-ray scattering is a technique for the characterization and structural analysis of a variety of materials including biological macromolecules and polymers. For the conformational analysis of proteins, the interaction between sample and X-rays is generally performed when the proteins are present in solution. Here a three-dimensional digital ion trap interfaced with a high intensity X-ray source is built to prove that X-ray scattering can be performed on ions isolated in gas-phase. Initial experiments on an unresolved ion population of multiply charged cytochrome C ions indicate that a small-angle X-ray scattering signal can be detected and that partial structural information can be extracted about the overall molecular structure of protein ions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Difração de Raios X
/
Biopolímeros
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Gases
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article