[Development of immunochromatographic test systems for express detection of plant viruses].
Prikl Biokhim Mikrobiol
; 45(2): 225-31, 2009.
Article
em Ru
| MEDLINE
| ID: mdl-19382712
ABSTRACT
Express immunochromatographic test-strip assays were developed for detection of five plant viruses varying in shape and size of virions spherical carnation mottle virus, bean mild mosaic virus, rod-shaped tobacco mosaic virus, and filamentous potato viruses X and Y. Multimembrane composites (test strips) with immobilized polyclonal antibodies against viruses and colloidal gold-conjugated antibodies were used for the analysis. The immunochromatographic test strips were shown to enable the detection of viruses both in purified preparations and in leaf extracts of infected plants with a sensitivity from 0.08 to 0.5 microg/ml for 10 min. The test strips may be used for express diagnostics of plant virus diseases in field conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de Plantas
/
Coloide de Ouro
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
Idioma:
Ru
Ano de publicação:
2009
Tipo de documento:
Article