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Rapid detection of point mutations in Plasmodium falciparum genes associated with antimalarial drugs resistance by using High-Resolution Melting analysis.
Andriantsoanirina, Valérie; Lascombes, Vincent; Ratsimbasoa, Arsène; Bouchier, Christiane; Hoffman, Jonathan; Tichit, Magali; Rabarijaona, Leon-Paul; Durand, Rémy; Ménard, Didier.
Afiliação
  • Andriantsoanirina V; Malaria Unit Research, Institut Pasteur de Madagascar, Antananarivo, Madagascar.
J Microbiol Methods ; 78(2): 165-70, 2009 Aug.
Article em En | MEDLINE | ID: mdl-19465064
We have developed a High-Resolution DNA Melting method to detect mutations related to Plasmodium falciparum resistance. This method is based on real-time PCR followed by High Resolution Melting ramping from 67 degrees C to 80 degrees C with fluorescence data acquisition set at 0.1 degrees C increments. The accuracy of the technique was assessed using 177 P. falciparum clinical isolates and two reference strains. Results perfectly matched those obtained by DNA sequencing for some important genetic markers of P. falciparum resistance. This technique could be of great value for epidemiological studies, especially in developing countries.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmodium falciparum / Resistência a Medicamentos / DNA de Protozoário / Mutação Puntual / Testes de Sensibilidade Parasitária / Temperatura de Transição / Antimaláricos Tipo de estudo: Diagnostic_studies / Evaluation_studies / Risk_factors_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmodium falciparum / Resistência a Medicamentos / DNA de Protozoário / Mutação Puntual / Testes de Sensibilidade Parasitária / Temperatura de Transição / Antimaláricos Tipo de estudo: Diagnostic_studies / Evaluation_studies / Risk_factors_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2009 Tipo de documento: Article