[Study on relationship between hepatitis B virus DNA load and genotype with large envelope protein].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 22(5): 348-50, 2008 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-19469171
ABSTRACT
OBJECTIVE:
To explore the relation between hepatitis B virus DNA load and genotype with the level of large envelope protein.METHODS:
Serum HBV DNA was quantitively detected by using real time polymerase chain reaction (RT-PCR). The LHBs were detected by using enzyme linked immuno sorbent assay (ELISA) and HBV markers were detected by time differentiate immunofluorescence assay in 140 serum samples collected from chronic hepatitis B patients.The genotypes of HBV were identified by DNA sequencing; and analyze their relationship.RESULTS:
There was no significant difference between positive rate of LHBs and that of HBV DNA in HBeAg negative and positive group (P > 0.05); The HBV LHBs absorbency was markedly correlated with the HBV DNA load ( R2 = 0.9267). The difference of HBV LHBs absorbency between HBV genotype B and C was not significant.CONCLUSIONS:
The close correlation between HBV LHBs absorbence and HBV DNA load illustrated that he level of serum LHBs can be used to estimate the state of HBV replication; and there is no relationship between HBV LHBs absorbency and genotypes. So HBV LHBs may be used as a new serological marker to detect HBV replication.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírion
/
DNA Viral
/
Vírus da Hepatite B
/
Genótipo
Limite:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article