X-ray spectromicroscopy study of protein adsorption to a polystyrene-polylactide blend.
Biomacromolecules
; 10(7): 1838-45, 2009 Jul 13.
Article
em En
| MEDLINE
| ID: mdl-19496614
ABSTRACT
Synchrotron-based X-ray photoemission electron microscopy (X-PEEM) was used to study the adsorption of human serum albumin (HSA) to polystyrene-polylactide (4060 PS-PLA, 0.7 wt %) thin films, annealed under various conditions. The rugosity of the substrate varied from 35 to 90 nm, depending on the annealing conditions. However, the characteristics of the protein adsorption (amounts and phase preference) were not affected by the changes in topography. The adsorption was also not changed by the phase inversion which occurred when the PS-PLA substrate was annealed above T(g) of the PLA. The amount of protein adsorbed depended on whether adsorption took place from distilled water or phosphate buffered saline solution. These differences are interpreted as a result of ionic strength induced changes in the protein conformation in solution.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Poliestirenos
/
Microscopia Eletrônica
/
Proteínas
/
Adsorção
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article