[Hepatitis B virus P22(e) inhibit hepatocyte apoptosis via nuclear factor kappa B].
Zhonghua Gan Zang Bing Za Zhi
; 17(5): 359-62, 2009 May.
Article
em Zh
| MEDLINE
| ID: mdl-19497202
OBJECTIVE: To test whether nuclear factor kappa B plays an important role in the apoptosis-inhibitory effect of hepatitis B virus (HBV) P22(e) protein. METHODS: HepG2 cells were transfected with recombination plasmid pEGFP-HBVP22(e). The Act-D and TNF alpha were used to induce apoptosis. NF-kappa B inhibitor ALLN were used to inhibit the signaling pathway. The activation of NF-kappa B was EMSA, and the nulear translocation of NF-kappa B was determined by immuno-staining. RESULTS: Laser scanning confocal microscopy and EMSA indicated that HBV P22(e) protein enhanced the nuclear translocation of NF-kappa B after apoptosis induction. ALLN treatment inhibited the nuclear translocation of NF-kappa B, and blocked the apoptosis-inhibiting effect of HBV P22(e) protein. CONCLUSION: This study indicates that HBV P22(e) protein inhibits apoptosis of hepatocyte via the NF-kappa B signaling pathway.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas do Core Viral
/
NF-kappa B
/
Apoptose
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Carcinoma Hepatocelular
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Antígenos do Núcleo do Vírus da Hepatite B
/
Neoplasias Hepáticas
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article