Production of flavin mononucleotide by metabolically engineered yeast Candida famata.
Metab Eng
; 11(3): 163-7, 2009 May.
Article
em En
| MEDLINE
| ID: mdl-19558965
ABSTRACT
Recombinant strains of the flavinogenic yeast Candida famata able to overproduce flavin mononucleotide (FMN) that contain FMN1 gene encoding riboflavin (RF) kinase driven by the strong constitutive promoter TEF1 (translation elongation factor 1alpha) were constructed. Transformation of these strains with the additional plasmid containing the FMN1 gene under the TEF1 promoter resulted in the 200-fold increase in the riboflavin kinase activity and 100-fold increase in FMN production as compared to the wild-type strain (last feature was found only in iron-deficient medium). Overexpression of the FMN1 gene in the mutant that has deregulated riboflavin biosynthesis pathway and high level of riboflavin production in iron-sufficient medium led to the 30-fold increase in the riboflavin kinase activity and 400-fold increase in FMN production of the resulted transformants. The obtained C. famata recombinant strains can be used for the further construction of improved FMN overproducers.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Candida
/
Proteínas Fúngicas
/
Fosfotransferases (Aceptor do Grupo Álcool)
/
Mononucleotídeo de Flavina
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article