Direct quantitation of the quorum sensing signal, autoinducer-2, in clinically relevant samples by liquid chromatography-tandem mass spectrometry.
Anal Chem
; 81(15): 6374-81, 2009 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-19594136
ABSTRACT
Quorum Sensing is a type of bacterial cell-to-cell signaling that allows for cell density dependent regulation of gene expression. Many of the behaviors mediated by quorum sensing are critical for bacterial colonization or infection, and autoinducer-2 has been proposed as a universal interspecies signaling molecule that allows multispecies colonies of bacteria, e.g., biofilms or dental plaque, to behave as pseudomulticellular organisms. However, the direct detection of autoinducer-2 has been difficult, leaving the in vivo relevance of this signal in question. Herein we report a liquid chromatography-tandem mass spectrometric technique that enables reproducible, quantitative, and sensitive measurement of the concentration of autoinducer-2 from a variety of sources. This technique was applied to the detection of autoinducer-2 from Escherichia coli and Vibrio harveyi in proof-of-concept studies and was then used to directly measure the concentration of the signal produced by oral bacteria in human saliva.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Transdução de Sinais
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Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas em Tandem
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Percepção de Quorum
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Homosserina
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Lactonas
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article