Single-step production of functional OEP24 proteoliposomes.
Protein Expr Purif
; 69(1): 106-11, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-19602439
ABSTRACT
The pea chloroplastic outer envelope protein OEP24 is a voltage-dependent channel that can function as a general solute channel in plants. OEP24 is a close functional homologue of VDAC which, in mammalian cells, modulates the permeability of the outer mitochondrial membrane. Here, we describe the production in a one-step reaction of active OEP24 in proteoliposomes or in soluble form using a cell-free expression system. We combine evidence from electrophysiological experiments, biophysical characterization, and biochemical analysis demonstrating that OEP24 is present as a functional channel in liposomes. Thus, production of OEP-containing proteoliposomes may provide a helpful tool for deciphering the role of the OEP family members.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Proteolipídeos
/
Biotecnologia
/
Canais Iônicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article