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Identification of a short Spir interaction sequence at the C-terminal end of formin subgroup proteins.
Pechlivanis, Markos; Samol, Annette; Kerkhoff, Eugen.
Afiliação
  • Pechlivanis M; Bavarian Genome Research Network (BayGene), Cell Structure and Genomics, Institute of Functional Genomics, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
J Biol Chem ; 284(37): 25324-33, 2009 Sep 11.
Article em En | MEDLINE | ID: mdl-19605360
The actin nucleation factors Spire and Cappuccino interact with each other and regulate essential cellular events during Drosophila oogenesis in a cooperative fashion. The interaction blocks formin actin nucleation activity and enhances the Spire activity. Analogous to Spire and Cappuccino, the mammalian homologs Spir-1 and formin-2 show a regulatory interaction. To get an understanding of the nature of the Spir-formin cooperation, we have analyzed the interaction biochemically and biophysically. Our data shows that the association of Spir-1 and formin-2 is not significantly mediated by binding of the Spir-1-KIND domain to the formin FH2 core domain. Instead, a short sequence motif C-terminal adjacent to the formin-2-FH2 domain could be characterized that mediates the interaction and is conserved among the members of the Fmn subgroup of formins. In line with this, we found that both mammalian Spir proteins, Spir-1 and Spir-2, interact with mammalian Fmn subgroup proteins formin-1 and formin-2.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Nucleares / Proteínas Fetais / Proteínas dos Microfilamentos / Proteínas do Tecido Nervoso Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Nucleares / Proteínas Fetais / Proteínas dos Microfilamentos / Proteínas do Tecido Nervoso Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2009 Tipo de documento: Article