[Androgens stimulate the expression of SOCS-3 and inhibits their effect on proliferation and secretion]. / C. E. Alken Preis 2007: Androgene stimulieren die Expression von SOCS-3 und bewirken die Inhibition der Proliferation und Sekretion.
Aktuelle Urol
; 40(4): 231-4, 2009 Aug.
Article
em De
| MEDLINE
| ID: mdl-19634073
ABSTRACT
PURPOSE:
Suppressors of cytokine signalling (SOCS) are induced by interleukins and peptide hormones. These molecules prevent the activation of diverse signalling pathways in benign and malignant cells. In previous studies, we showed that SOCS-3 is expressed in most prostate cancer cell lines and tissue specimens. In the present study we investigated the effects of androgen on the regulation of SOCS-3 in prostate cancer cell lines. MATERIALS ANDMETHODS:
SOCS-3 expression was determined with PCR and Western blot techniques. The activity of the SOCS-3 promoter was measured with the luciferase test. We measured proliferation with (3)H-thymidine assay.RESULTS:
We show that androgen induces the expression of SOCS-3 in two prostate cancer cell lines. The non-steroidal anti-androgen bicalutamide is able to block the induction of SOCS-3 -expression. Androgenic hormones did not induce the expression of SOCS-3 mRNA or its promoter activity. In LNCaP-IL-6- cells transfected with the inducible Tet-On construct SOCS-3 expression was induced. The effects of androgenic hormones on the proliferation and induction of PSA were -diminished in the presence of SOCS-3.CONCLUSIONS:
Our results show that androgenic -regulation of SOCS-3 leads to inhibition of prolif-eration and secretion in human prostate cancer.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
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Compostos de Tosil
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Expressão Gênica
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Divisão Celular
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Regiões Promotoras Genéticas
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Proteínas Supressoras da Sinalização de Citocina
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Antagonistas de Androgênios
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Androgênios
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Anilidas
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Nitrilas
Limite:
Humans
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Male
Idioma:
De
Ano de publicação:
2009
Tipo de documento:
Article