Production of Clostridium difficile toxin in a medium totally free of both animal and dairy proteins or digests.
Proc Natl Acad Sci U S A
; 106(32): 13225-9, 2009 Aug 11.
Article
em En
| MEDLINE
| ID: mdl-19651616
ABSTRACT
In the hope of developing a vaccine against Clostridium difficile based on its toxin(s), we have developed a fermentation medium for the bacterium that results in the formation of Toxin A and contains no meat or dairy products, thus obviating the problem of possible prion diseases. Particular preparations of hydrolyzed soy proteins, especially Soy Peptone A3, have been found to replace both the meat/dairy product tryptone in the preparation of working cell banks and seed media, and NZ-Soy BL4 does the same in the fermentation medium. These replacements yield even higher toxin titers.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Proteínas
/
Clostridioides difficile
/
Meios de Cultura
/
Laticínios
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article