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A whole-genome phylogeny of the family Pasteurellaceae.
Bonaventura, Maria Pia Di; Lee, Ernest K; Desalle, Rob; Planet, Paul J.
Afiliação
  • Bonaventura MP; Sackler Institute of Comparative Genomics, American Museum of Natural History, 79th Street at Central Park West, New York, NY 10024, USA.
Mol Phylogenet Evol ; 54(3): 950-6, 2010 Mar.
Article em En | MEDLINE | ID: mdl-19686857
ABSTRACT
A phylogenomic approach was used to generate an amino acid phylogeny for 12 whole genomes representing 10 species in the family Pasteurellaceae. Orthology of genes was determined using an approach similar to OrthologID (http//nypg.bio.nyu.edu/orthologid/about.html) and resulted in the generation of a matrix with 3130 genes with 1,194,615 aligned amino acid characters of which 239,504 characters are phylogenetically informative. Phylogenetic analysis of the concatenated matrix using all standard approaches (maximum parsimony, maximum likelihood, and Bayesian analysis) results in a single extremely robust phylogenetic hypothesis for the species examined in this study. Remarkably, no single gene partition gives the same tree as the concatenated analysis. By analyzing partitioned support in the data matrix, we show that there is very little negative support emanating from individual gene partitions to suggest that the concatenated hypothesis is not tenable. The large number of characters in the matrix allows us to test hypotheses concerning missing data and character number in phylogenomic studies, and we conclude that matrices constructed using genome level information are very robust to missing data. We show that a very large number of concatenated gene sequences (>160) are needed to reliably obtain the same topology as the overall analysis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Pasteurellaceae / Genoma Bacteriano / Modelos Genéticos Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Pasteurellaceae / Genoma Bacteriano / Modelos Genéticos Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2010 Tipo de documento: Article