Myosin-X is required for cranial neural crest cell migration in Xenopus laevis.
Dev Dyn
; 238(10): 2522-9, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-19718754
ABSTRACT
Myosin-X (MyoX) belongs to a large family of unconventional, nonmuscle, actin-dependent motor proteins. We show that MyoX is predominantly expressed in cranial neural crest (CNC) cells in embryos of Xenopus laevis and is required for head and jaw cartilage development. Knockdown of MyoX expression using antisense morpholino oligonucleotides resulted in retarded migration of CNC cells into the pharyngeal arches, leading to subsequent hypoplasia of cartilage and inhibited outgrowth of the CNC-derived trigeminal nerve. In vitro migration assays on fibronectin using explanted CNC cells showed significant inhibition of filopodia formation, cell attachment, spreading and migration, accompanied by disruption of the actin cytoskeleton. These data support the conclusion that MyoX has an essential function in CNC migration in the vertebrate embryo.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xenopus laevis
/
Movimento Celular
/
Miosinas
/
Proteínas de Xenopus
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Crista Neural
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article