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Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques.
Capelo, J L; Carreira, R; Diniz, M; Fernandes, L; Galesio, M; Lodeiro, C; Santos, H M; Vale, G.
Afiliação
  • Capelo JL; Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, University of Vigo at Ourense Campus, 32004, Ourense, Spain. jlcapelom@uvigo.es
Anal Chim Acta ; 650(2): 151-9, 2009 Sep 21.
Article em En | MEDLINE | ID: mdl-19720186
ABSTRACT
Recent tools addressed to accelerate the different steps of the sample treatment for protein identification in modern workflows are reviewed and critically commented in this manuscript. Heating, microspin columns, ultrasonic energy, high pressure, infrared energy, microwave energy, alternating electric fields and microreactors are outlined as useful tools that can be used to accelerate all or some of the following steps for in-gel or in-liquid based approaches for protein identification (i) protein dissolution/denaturation, (ii) protein reduction, (iii) protein alkylation and (iv) protein digestion. The advantages and drawbacks, along with the main differences among the different tools are also commented. Future prospects for hyphenation of methods are also discussed. Researchers are informed also in this work regarding the main problems to be found when implementing any of the above mentioned methods.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Proteínas / Enzimas Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Proteínas / Enzimas Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2009 Tipo de documento: Article