EDG3 and SHC3 on chromosome 9q22 are co-amplified in human ependymomas.
Cancer Lett
; 290(1): 36-42, 2010 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-19748727
ABSTRACT
By qPCR we found that EDG3 and SHC3 were amplified in 60% of ependymomas but none in choroid plexus papillomas. In ependymomas EDG3 and SHC3 amplification increased Shc3 protein levels while EDG3 was less affected. Both proteins were co-immunoprecipitated from ependymoma and Shc3 was tyrosine phosphorylated thus presumably active. We showed by digestion with N-glycosidase-F that EDG3 was glycosylated indicating that EDG3 protein was not retained in the endoplasmic reticulum. The co-immunoprecipitation of Shc3 and EDG3 proteins from ependymomas with amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 9
/
Receptores de Lisoesfingolipídeo
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Ependimoma
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Proteínas Adaptadoras da Sinalização Shc
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article