Cloning, expression, and characterization of a dithiol glutaredoxin from Trypanosoma cruzi.
Antioxid Redox Signal
; 12(6): 787-92, 2010 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-19769456
ABSTRACT
Glutaredoxins play an important role in cellular functionality. A putative dithiol glutaredoxin is encoded in the genome of Trypanosoma cruzi. We cloned the gene and obtained the recombinant protein, which behaved as a typical thioltransferase. Activity was variable and dependent on the nature of reducer or oxidant agent used, or both. Epimastigote extracts exhibited similar activity, suggesting the occurrence of the protein in the parasite. Results support a redox scenario in T. cruzi, with glutaredoxin being involved mainly in reduction of glutathione disulfide as well as in deglutathionylation of target proteins.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Glutarredoxinas
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article