Induction of B-cell development in adult mice reveals the ability of bone marrow to produce B-1a cells.
Blood
; 114(24): 4960-7, 2009 Dec 03.
Article
em En
| MEDLINE
| ID: mdl-19812384
ABSTRACT
To study B-cell development from bone marrow (BM), we generated recombination-activating gene 1 (Rag1)-targeted mice lacking mature lymphocytes. B-cell development can be induced in such mice by B cell-specific restoration of a functional Rag1 transcription unit. Follicular and marginal zone B cells populated the spleen when Rag1 expression was permitted. Notably, the peritoneal cavity was dominated by bona fide B-1a cells, as judged by surface markers and functional properties. These BM-derived B-1a cells exhibited a polyclonal VDJ repertoire with substantial N nucleotide insertions. Nevertheless, physiologic frequencies of phosphatidylcholine-specific B cells were detected. Importantly, the BM of young and 5-month-old mice was indistinguishable with regard to the potential to generate B-1a cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
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Linfócitos B
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Diferenciação Celular
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Subpopulações de Linfócitos B
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article