Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells.
BMB Rep
; 42(10): 691-6, 2009 Oct 31.
Article
em En
| MEDLINE
| ID: mdl-19874716
ABSTRACT
The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação Transcricional
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Fator de Transcrição E2F1
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article