Microfluidic analysis of complex samples with minimal sample preparation using gradient elution moving boundary electrophoresis.
Anal Chem
; 81(24): 10201-7, 2009 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-19902932
ABSTRACT
Sample-in answer-out analytical tools remain the goal of much lab on a chip research, but miniaturized methods capable of examining minimally prepared samples have proven elusive. Complex samples, including whole milk, various types of dirt and leaves, coal fly ash, and blood serum, were analyzed quantitatively for dissolved potassium, calcium, sodium, magnesium, lithium, and melamine using gradient elution moving boundary electrophoresis (GEMBE) and contactless conductivity detection with the single preparatory step of dilution or suspension in sample buffer. GEMBE is a simple, robust analytical technique, well-suited to microfluidic analysis of complex samples containing material, such as particulates or proteins, that would confound the majority of other microfluidic techniques. GEMBE utilizes electrophoretic flow to drive electrically charged analytes into a microfluidic channel or capillary for detection, while opposing electro-osmotic and variable pressure-driven flows prevent the remainder of the sample from entering the channel. Contactless conductivity detection further simplifies device construction and operation, positioning GEMBE for inexpensive and facile multiplexed implementation outside laboratory settings.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbono
/
Folhas de Planta
/
Soro
/
Leite
/
Técnicas Analíticas Microfluídicas
/
Material Particulado
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article