Biodegradable thermosensitive injectable PEG-PCL-PEG hydrogel for bFGF antigen delivery to improve humoral immunity.
Growth Factors
; 27(6): 377-83, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-19919526
ABSTRACT
In this contribution, a biodegradable and injectable thermosensitive poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel system was successfully prepared for basic fibroblastic growth factor (bFGF) antigen delivery. bFGF encapsulated PECE hydrogel system (bFGF-hydrogel) is an injectable free-flowing sol at ambient temperature, and forms a non-flowing gel at physiological temperature acting as antigen depot. Furthermore, the cytotoxicity results showed that the PECE hydrogel could be regarded as a safe carrier, and bFGF could be released from the hydrogel system in an extended period in vitro. Otherwise, the immunogenicity of bFGF was improved significantly after encapsulated into the hydrogel. Strong humoral immunity created by bFGF-hydrogel was maintained for more than 14 weeks. Therefore, the prepared bFGF loaded PECE hydrogel might have great potential as a novel vaccine adjuvant for protein antigen.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Polietilenoglicóis
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Fator 2 de Crescimento de Fibroblastos
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Sistemas de Liberação de Medicamentos
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Hidrogel de Polietilenoglicol-Dimetacrilato
/
Antígenos
Tipo de estudo:
Evaluation_studies
Limite:
Animals
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Female
/
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article