The ubiquitous transcription factor Oct-1 and the liver-specific factor HNF-1 are both required to activate transcription of a hepatitis B virus promoter.
Mol Cell Biol
; 11(3): 1353-9, 1991 Mar.
Article
em En
| MEDLINE
| ID: mdl-1996097
ABSTRACT
The liver-specific transcription factor HNF-1 activates transcription of several mammalian hepatocyte-specific genes. The hepatitis B virus preS1 promoter shows hepatocyte specificity, which has been ascribed to binding of HNF-1 to a cognate DNA sequence upstream of the TATA box. We show here that there is an adjacent site that binds the ubiquitous transcription factor Oct-1. Both the Oct-1 and HNF-1 sites are necessary for liver-specific transcription of the preS1 promoter, but neither site alone activates transcription. The Oct-1 site is also necessary for activation of the preS1 promoter in HeLa cells, expressing transfected HNF-1. Our results show that while Oct-1 is not restricted to hepatocytes, it nevertheless can play a critical role in the expression of a liver-specific gene.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Precursores de Proteínas
/
Fatores de Transcrição
/
Proteínas Nucleares
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Regulação Viral da Expressão Gênica
/
Sequências Reguladoras de Ácido Nucleico
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Vírus da Hepatite B
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Proteínas de Ligação a DNA
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Antígenos de Superfície da Hepatite B
Limite:
Humans
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article